Sedimentation Analyses of Polyribosomes
نویسندگان
چکیده
منابع مشابه
Drosophila Polyribosomes
Two populations of polyribosomes have been isolated from third instar larvae of D. melanogaster. One population appeared to be soluble while the second seemed membrane-bound. Short-term labeling of the two RNP fractions with radioactive nucleic acid and protein precursors was achieved by using a feeding stimulant. RNA was extracted from both polyribosomal fractions following 25, 40, and 60 min ...
متن کاملPolyribosomes of Escherichia coli
Growing cells incubated in a medium lacking glucose undergo a rapid breakdown of polyribosomes to single ‘70 S ribosomes. This conversion is accompanied by a loss of protein-synthetic capacity in vivo and is inhibited by chloramphenicol, but not by puromycin. The conversion of polysomes to 70 S ribosomes, with a half-life of 1: to 24 min at 37’, appears to reflect a breakdown of messenger ribon...
متن کاملPolyribosomes of Escherichia coli
Growing cells incubated in a medium lacking glucose undergo a rapid breakdown of polyribosomes to single ‘70 S ribosomes. This conversion is accompanied by a loss of protein-synthetic capacity in vivo and is inhibited by chloramphenicol, but not by puromycin. The conversion of polysomes to 70 S ribosomes, with a half-life of 1: to 24 min at 37’, appears to reflect a breakdown of messenger ribon...
متن کاملPolyribosomes from Tetrahymena
The free-living, ciliated protist Tetrahymena, which can be grown in synchronized cultures, lends itself to studies of protein synthesis and organelle morphogenesis in eukaryotic cells . The isolation of polyribosomes, the sites of protein synthesis, from Tetrahymena was reported by Whitson et al . (5) . After lysis of the cells by indole-saturated buffer, the polysomes were isolated in 0 .01 M...
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Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...
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ژورنال
عنوان ژورنال: The Journal of Kansai Medical University
سال: 1969
ISSN: 0022-8400
DOI: 10.5361/jkmu1956.21.4_138